rabbit anti mouse polyclonal antibody sirt1  (Bioss)

Journal: American Journal of Translational Research

Article Title: SIRT1 ameliorates renal ischemia-reperfusion injury through suppressing endoplasmic reticulum stress-mediated autophagy

doi:

Figure Lengend Snippet: SIRT1 upregulation preserves renal function in kidneys with IR injury. A. SIRT1 protein levels were measured by western blot analysis at different reperfusion time points. B. Statistical analysis of SIRT1 expression. C. Serum BUN and SCr levels in the IR, IR+SRT1720, and IR+selisistat groups at 0, 6, 12, 24, and 48 h postischemia. D. H&E staining of kidney tissues 24 h postreperfusion (20×) and tubular injury scores were assessed. Scale bar represent 20 μm. The data are shown as the mean ± SD. N = 6 for each group. *P < 0.05, **P < 0.01.

Article Snippet: Next, the membrane was blocked with 5% nonfat milk for 1 h and then incubated overnight at 4°C with the following specific primary antibodies: polyclonal rabbit anti-mouse SIRT1, BiP, CHOP, p-PERK, PERK, p-IRE1α, IRE1α, ATF6, Atg-7, Beclin-1, LC3B, and GAPDH Cell Signaling Technology, Danvers, MA, USA.

Techniques: Western Blot, Expressing, Staining

Journal: American Journal of Translational Research

Article Title: SIRT1 ameliorates renal ischemia-reperfusion injury through suppressing endoplasmic reticulum stress-mediated autophagy

doi:

Figure Lengend Snippet: SIRT1 activation reduces the IR-induced inflammatory milieu. A. Histograms of the mRNA expression levels of TNF-α, IL-6, and IL-10. B. Representative immunofluorescence of MPO-stained and DAPI-stained kidney sections. C. Analysis of MPO-positive cells. Scale bar represents 20 μm. The data are expressed as the mean ± SD. N = 6 for each group. *P < 0.05, **P < 0.01.

Article Snippet: Next, the membrane was blocked with 5% nonfat milk for 1 h and then incubated overnight at 4°C with the following specific primary antibodies: polyclonal rabbit anti-mouse SIRT1, BiP, CHOP, p-PERK, PERK, p-IRE1α, IRE1α, ATF6, Atg-7, Beclin-1, LC3B, and GAPDH Cell Signaling Technology, Danvers, MA, USA.

Techniques: Activation Assay, Expressing, Immunofluorescence, Staining

Journal: American Journal of Translational Research

Article Title: SIRT1 ameliorates renal ischemia-reperfusion injury through suppressing endoplasmic reticulum stress-mediated autophagy

doi:

Figure Lengend Snippet: SIRT1 enhancement attenuates IR-induced ER stress. A. Representative photographs of immunohistochemical staining of BiP and CHOP. B. Quantitative analysis of BiP staining. C. Quantitative analysis of CHOP staining. D. Protein expression levels of BiP, CHOP, p-PERK, PERK, p-IRE1α, IRE1α, ATF6, and GAPDH were measured by western blotting. E-I. Quantitative statistical analysis of protein expression. Scale bar represents 50 μm. The data are presented as mean ± SD. N = 6 for each group. *P < 0.05.

Article Snippet: Next, the membrane was blocked with 5% nonfat milk for 1 h and then incubated overnight at 4°C with the following specific primary antibodies: polyclonal rabbit anti-mouse SIRT1, BiP, CHOP, p-PERK, PERK, p-IRE1α, IRE1α, ATF6, Atg-7, Beclin-1, LC3B, and GAPDH Cell Signaling Technology, Danvers, MA, USA.

Techniques: Immunohistochemical staining, Staining, Expressing, Western Blot

Journal: American Journal of Translational Research

Article Title: SIRT1 ameliorates renal ischemia-reperfusion injury through suppressing endoplasmic reticulum stress-mediated autophagy

doi:

Figure Lengend Snippet: SIRT1 upregulation reduces renal ER stress-induced autophagy. A. The expression levels of BiP, CHOP, Atg-7, Beclin-1, LC3, and GAPDH were assessed by western blotting. B-F. Quantitative analysis of BiP, CHOP, Atg-7, Beclin-1, and LC3 II. G. Representative immunofluorescence images of LC3-stained sections and statistical analysis of LC3-positive cells. Scale bar represents 20 μm. The data are expressed as mean ± SD. N = 6 for each group. *P < 0.05, **P < 0.01, n.s.>0.05.

Article Snippet: Next, the membrane was blocked with 5% nonfat milk for 1 h and then incubated overnight at 4°C with the following specific primary antibodies: polyclonal rabbit anti-mouse SIRT1, BiP, CHOP, p-PERK, PERK, p-IRE1α, IRE1α, ATF6, Atg-7, Beclin-1, LC3B, and GAPDH Cell Signaling Technology, Danvers, MA, USA.

Techniques: Expressing, Western Blot, Immunofluorescence, Staining

Journal: Cell reports

Article Title: Interplay between protein acetylation and ubiquitination controls MCL1 protein stability

doi: 10.1016/j.celrep.2021.109988

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-SirT1 (1F3) , Cell Signaling Technology , Cat# 8469, RRID: AB_10999470.

Techniques: Purification, Virus, Recombinant, Mutagenesis, Extraction, Cell Fractionation, Mass Spectrometry, Western Blot, CRISPR, Software

Journal: Experimental and Therapeutic Medicine

Article Title: Ginsenoside Rg1 protects against Sca-1 + HSC/HPC cell aging by regulating the SIRT1-FOXO3 and SIRT3-SOD2 signaling pathways in a γ-ray irradiation-induced aging mice model

doi: 10.3892/etm.2020.8810

Figure Lengend Snippet: Primers used for quantitative PCR.

Article Snippet: Then, the PVDF membranes were incubated with rabbit anti-mouse SIRT1 polyclonal antibody (cat. no. sc-15404, 1:3,000; Santa Cruz Biotechnology, Inc.), rabbit anti-mouse SIRT3 polyclonal antibody (cat. no. sc-99143, 1:3,000; Santa Cruz Biotechnology, Inc.), rabbit anti-mouse FOXO3 polyclonal antibody (cat. no. ab47285, 1:3,000; Abcam), rabbit anti-mouse SOD2 polyclonal antibody (cat. no. sc-30080; 1:2,000; Santa Cruz Biotechnology, Inc.) and rabbit anti-mouse GAPDH polyclonal antibody (cat. no. sc-25778; 1:2,000; Santa Cruz Biotechnology, Inc.) for 2 h at room temperature.

Techniques:

Journal: Experimental and Therapeutic Medicine

Article Title: Ginsenoside Rg1 protects against Sca-1 + HSC/HPC cell aging by regulating the SIRT1-FOXO3 and SIRT3-SOD2 signaling pathways in a γ-ray irradiation-induced aging mice model

doi: 10.3892/etm.2020.8810

Figure Lengend Snippet: Effects of Rg1 treatment on SIRT1, SIRT3, FOXO3 and SOD2 mRNA expression in Sca-1 + HSC/HPCs. Relative mRNA expression of (A) SIRT1, (B) SIRT3, (C) FOXO3 and (D) SOD2. Results are presented as mean ± SD (n=6). ** P<0.01 vs. the control group; # P<0.05 vs. the γ-ray irradiation group. Rg1, ginsenoside Rg1; SIRT, sirtuin; FOXO3, forkhead box O3; SOD, superoxide dismutase 2; Sca-1 + , stem cell antigen 1 positive; HSC, hematopoietic stem cell; HPC, hematopoietic progenitor cell.

Article Snippet: Then, the PVDF membranes were incubated with rabbit anti-mouse SIRT1 polyclonal antibody (cat. no. sc-15404, 1:3,000; Santa Cruz Biotechnology, Inc.), rabbit anti-mouse SIRT3 polyclonal antibody (cat. no. sc-99143, 1:3,000; Santa Cruz Biotechnology, Inc.), rabbit anti-mouse FOXO3 polyclonal antibody (cat. no. ab47285, 1:3,000; Abcam), rabbit anti-mouse SOD2 polyclonal antibody (cat. no. sc-30080; 1:2,000; Santa Cruz Biotechnology, Inc.) and rabbit anti-mouse GAPDH polyclonal antibody (cat. no. sc-25778; 1:2,000; Santa Cruz Biotechnology, Inc.) for 2 h at room temperature.

Techniques: Expressing, Control, Irradiation

Journal: Experimental and Therapeutic Medicine

Article Title: Ginsenoside Rg1 protects against Sca-1 + HSC/HPC cell aging by regulating the SIRT1-FOXO3 and SIRT3-SOD2 signaling pathways in a γ-ray irradiation-induced aging mice model

doi: 10.3892/etm.2020.8810

Figure Lengend Snippet: Effects of Rg1 treatment on SIRT1, SIRT3, FOXO3 and SOD2 protein expression in Sca-1 + HSC/HPCs. (A) Western blot bands for SIRT1, SIRT3, FOXO3 and SOD2. (B) Quantification of relative SIRT1, SIRT3, FOXO3 and SOD2 expression. Results are presented as mean ± SD (n=6). ** P<0.01 vs. the control group; # P<0.05 vs. the γ-ray irradiation group. Rg1, ginsenoside Rg1; SIRT, sirtuin; FOXO3, forkhead box O3; SOD, superoxide dismutase 2; Sca-1 + , stem cell antigen 1 positive; HSC, hematopoietic stem cell; HPC, hematopoietic progenitor cell.

Article Snippet: Then, the PVDF membranes were incubated with rabbit anti-mouse SIRT1 polyclonal antibody (cat. no. sc-15404, 1:3,000; Santa Cruz Biotechnology, Inc.), rabbit anti-mouse SIRT3 polyclonal antibody (cat. no. sc-99143, 1:3,000; Santa Cruz Biotechnology, Inc.), rabbit anti-mouse FOXO3 polyclonal antibody (cat. no. ab47285, 1:3,000; Abcam), rabbit anti-mouse SOD2 polyclonal antibody (cat. no. sc-30080; 1:2,000; Santa Cruz Biotechnology, Inc.) and rabbit anti-mouse GAPDH polyclonal antibody (cat. no. sc-25778; 1:2,000; Santa Cruz Biotechnology, Inc.) for 2 h at room temperature.

Techniques: Expressing, Western Blot, Control, Irradiation

Journal: Frontiers in Pharmacology

Article Title: Quercetin Attenuates Atherosclerosis via Modulating Oxidized LDL-Induced Endothelial Cellular Senescence

doi: 10.3389/fphar.2020.00512

Figure Lengend Snippet: Quercetin alleviated atherosclerotic lesion in vivo . (A) Oil red O staining of entire arterial tree to assess lipid deposition and atherosclerotic lesion of mice (n = 3). (B) Quantification of the gross lesion areas in the entire arterial tree (n = 3). (C) Serum soluble Icam-1 level was determined by enzyme-linked immunosorbent assay (ELISA) assay (n = 7). (D) Serum IL-6 level was determined by ELISA assay (n = 7). (E) the representative photograph and semi-quantitative analysis of IHC of density of Vcam-1 and Sirt1 in aorta (n = 3). The sites in brown-yellow colour were judged as positive (× 400). * P < 0.05, vs C57 mice; Δ P < 0.05, vs ApoE -/- mice.

Article Snippet: Briefly, after 3% H 2 O 2 incubating for elimination of endogenous peroxidases, microwaving for antigen retrieval and 5% BSA blocking, sections were incubated with rabbit anti-mouse Vcam-1 primary antibody (1:800 dilution; Proteintech, 11444-1-AP, Wuhan, China) or rabbit anti-mouse Sirt1 primary antibody (1:100 dilution; Proteintech, 13161-1-AP, Wuhan, China) in a moisture box at 4°C overnight.

Techniques: In Vivo, Staining, Enzyme-linked Immunosorbent Assay